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KMID : 1007520210300131645
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Food Science and Biotechnology
2021 Volume.30 No. 13 p.1645 ~ p.1655
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An improved extraction and purification method for obtaining high-quality chitin and chitosan from blue swimmer (Portunus pelagicus) crab shell waste
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Tissera Chethana Minoli
Rathnayake Suramya Indunil
Abeyrathne Sandun
Nam Ki-Chang
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Abstract
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Portunus pelagicus shell waste is highly accumulated in seafood processing factories and has low commercial applications. The objective of this study was to modify and develop a scale-up, simple, and high-yielding chemical method for extraction and purification of chitin and chitosan from P. pelagicus shell waste. The developed method included a new “pretreatment” process using acetic acid followed by chemical treatments at each purification step. The final product was characterized by XRD and FTIR spectroscopy. Control chitin and chitosan were produced using a pre-described method for comparison. Yields of crude chitin, chitosan, and purified chitosan were 32.52?±?0.68%, 26.28?±?0.47%, and 21.78?±?0.34% respectively whereas in the control chitin and chitosan the yields were 20.34?±?0.72% and 13.79?±?0.93% respectively (p?
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KEYWORD
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Crab shell, Chitosan, Pre-treatment, Purity, Physicochemical properties
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